BCHM 482 Proteomics and Metabolomics : Integrative Analysis

Get 30% Off + $20 Signup Bonus ! Limited Time, Hurry !

Securing Higher Grades Costing Your Pocket?
Book Your Assignment at The Lowest Price
Now!

Students Who Viewed This Also Studied

BCHM 482 Proteomics and Metabolomics

Task :

Paper #1: Plasma proteomic analysis reveals altered protein abundances in cardiovascular disease

Background

Cardiovascular disease (CVD) describes the pathological conditions of the heart and blood vessels. Despite the large number of studies on CVD and its aetiology, its key modulators remain largely unknown. To this end, we performed a comprehensive proteomic analysis of blood plasma, with the scope to identify disease-associated changes after placing them in the context of existing knowledge, and generate a well characterised dataset for further use in CVD multi-omics integrative analysis.

Methods

LC–MS/MS was employed to analyse plasma from 32 subjects (19 cases of various CVD phenotypes and 13 controls) in two steps: discovery (13 cases and 8 controls) and test (6 cases and 5 controls) set analysis. Following label-free quantification, the detected proteins were correlated to existing plasma proteomics datasets (plasma proteome database; PPD) and functionally annotated (Cytoscape, Ingenuity Pathway Analysis). Differential expression was defined based on identification confidence (≥ 2 peptides per protein), statistical significance (Mann–Whitney p value ≤ 0.05) and a minimum of twofold change.

Results

Peptides detected in at least 50% of samples per group were considered, resulting in a total of 3796 identified proteins (838 proteins based on ≥ 2 peptides). Pathway annotation confirmed the functional relevance of the findings (representation of complement cascade, fibrin clot formation, platelet degranulation, etc.). Correlation of the relative abundance of the proteins identified in the discovery set with their reported concentrations in the PPD was significant, confirming the validity of the quantification method. The discovery set analysis revealed 100 differentially expressed proteins between cases and controls, 39 of which were verified (≥ twofold change) in the test set. These included proteins already studied in the context of CVD (such as apolipoprotein B, alpha-2-macroglobulin), as well as novel findings (such as low density lipoprotein receptor related protein 2 [LRP2], protein SZT2) for which a mechanism of action is suggested.

Conclusions

This proteomic study provides a comprehensive dataset to be used for integrative and functional studies in the field. The observed protein changes reflect known CVD-related processes (e.g. lipid uptake, inflammation) but also novel hypotheses for further investigation including a potential pleiotropic role of LPR2 but also links of SZT2 to CVD.

Paper #2: Association of Circulating Metabolites With Risk of Coronary Heart Disease in a European Population

Task:

Importance:

Risk stratification for coronary heart disease (CHD) remains challenging because of the complex causative mechanism of the disease. Metabolomic profiling offers the potential to detect new biomarkers and improve CHD risk assessment.

Objective:  

To evaluate the association between circulating metabolites and incident CHD in a large European cohort.

Design, Setting, and Participants:  

This population-based study used the Biomarkers for Cardiovascular Risk Assessment in Europe (BiomarCaRE) case-cohort to measure circulating metabolites using a targeted approach in serum samples from 10 741 individuals without prevalent CHD. The cohort consisted of a weighted, random subcohort of the original cohort of more than 70 000 individuals. The case-cohort design was applied to 6 European cohorts: FINRISK97 (Finland), Monitoring of Trends and Determinants in Cardiovascular Diseases/Cooperative Health Research in the Region of Augsburg (MONICA/KORA; Germany), MONICA-Brianza and Moli-sani (Italy), DanMONICA (Denmark), and the Scottish Heart Health Extended Cohort (United Kingdom).

Main Outcomes and Measures:  

Associations with time to CHD onset were assessed individually by applying weighted and adjusted Cox proportional hazard models. The association of metabolites with CHD onset was examined by C indices.

Conclusions and Relevance:

Five PCs were significantly associated with increased risk of incident CHD and showed comparable discrimination with individual classic risk factors. Although these metabolites do not improve CHD risk assessment beyond that of classic risk factors, these findings hold promise for an improved understanding of the pathophysiology of CHD.

Paper #3: Large-scale Metabolomic Analysis Reveals Potential Biomarkers for Early Stage Coronary Atherosclerosis

https://www.nature.com/articles/s41598-017-12254-1 

Abstract:

Coronary atherosclerosis (CAS) is the pathogenesis of coronary heart disease, which is a prevalent and chronic life-threatening disease. Initially, this disease is not always detected until a patient presents with seriously vascular occlusion. Therefore, new biomarkers for appropriate and timely diagnosis of early CAS are needed for screening to initiate therapy on time. In this study, we used an untargeted metabolomics approach to identify potential biomarkers that could enable highly sensitive and specific CAS detection. Score plots from partial least-squares discriminant analysis clearly separated early-stage CAS patients from controls. Meanwhile, the levels of 24 metabolites increased greatly and those of 18 metabolites decreased markedly in early CAS patients compared with the controls, which suggested significant metabolic dysfunction in phospholipid, sphingolipid, and fatty acid metabolism in the patients. Furthermore, binary logistic regression showed that nine metabolites could be used as a combinatorial biomarker to distinguish early-stage CAS patients from controls. The panel of nine metabolites was then tested with an independent cohort of samples, which also yielded satisfactory diagnostic accuracy (AUC = 0.890). In conclusion, our findings provide insight into the pathological mechanism of early-stage CAS and also supply a combinatorial biomarker to aid clinical diagnosis of early-stage CAS.

Group Contract/Tasks for Presentation Slides:

Maria: summarise paper 1, intro

Tia: summarise paper 2, conclusion

Gabrielle: summarise paper 3, application

Rajesh: Compare and contrast the papers, identify gaps

BCHM 482 Proteomics and Metabolomics

Answer in Detail


Solved by qualified expert

Get Access to This Answer

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Hac habitasse platea dictumst vestibulum rhoncus est pellentesque. Amet dictum sit amet justo donec enim diam vulputate ut. Neque convallis a cras semper auctor neque vitae. Elit at imperdiet dui accumsan. Nisl condimentum id venenatis a condimentum vitae sapien pellentesque. Imperdiet massa tincidunt nunc pulvinar sapien et ligula. Malesuada fames ac turpis egestas maecenas pharetra convallis posuere. Et ultrices neque ornare aenean euismod. Suscipit tellus mauris a diam maecenas sed enim. Potenti nullam ac tortor vitae purus faucibus ornare. Morbi tristique senectus et netus et malesuada. Morbi tristique senectus et netus et malesuada. Tellus pellentesque eu tincidunt tortor aliquam. Sit amet purus gravida quis blandit. Nec feugiat in fermentum posuere urna. Vel orci porta non pulvinar neque laoreet suspendisse interdum. Ultricies tristique nulla aliquet enim tortor at auctor urna. Orci sagittis eu volutpat odio facilisis mauris sit amet.

Tellus molestie nunc non blandit massa enim nec dui. Tellus molestie nunc non blandit massa enim nec dui. Ac tortor vitae purus faucibus ornare suspendisse sed nisi. Pharetra et ultrices neque ornare aenean euismod. Pretium viverra suspendisse potenti nullam ac tortor vitae. Morbi quis commodo odio aenean sed. At consectetur lorem donec massa sapien faucibus et. Nisi quis eleifend quam adipiscing vitae proin sagittis nisl rhoncus. Duis at tellus at urna condimentum mattis pellentesque. Vivamus at augue eget arcu dictum varius duis at. Justo donec enim diam vulputate ut. Blandit libero volutpat sed cras ornare arcu. Ac felis donec et odio pellentesque diam volutpat commodo. Convallis a cras semper auctor neque. Tempus iaculis urna id volutpat lacus. Tortor consequat id porta nibh.

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Hac habitasse platea dictumst vestibulum rhoncus est pellentesque. Amet dictum sit amet justo donec enim diam vulputate ut. Neque convallis a cras semper auctor neque vitae. Elit at imperdiet dui accumsan. Nisl condimentum id venenatis a condimentum vitae sapien pellentesque. Imperdiet massa tincidunt nunc pulvinar sapien et ligula. Malesuada fames ac turpis egestas maecenas pharetra convallis posuere. Et ultrices neque ornare aenean euismod. Suscipit tellus mauris a diam maecenas sed enim. Potenti nullam ac tortor vitae purus faucibus ornare. Morbi tristique senectus et netus et malesuada. Morbi tristique senectus et netus et malesuada. Tellus pellentesque eu tincidunt tortor aliquam. Sit amet purus gravida quis blandit. Nec feugiat in fermentum posuere urna. Vel orci porta non pulvinar neque laoreet suspendisse interdum. Ultricies tristique nulla aliquet enim tortor at auctor urna. Orci sagittis eu volutpat odio facilisis mauris sit amet.

Tellus molestie nunc non blandit massa enim nec dui. Tellus molestie nunc non blandit massa enim nec dui. Ac tortor vitae purus faucibus ornare suspendisse sed nisi. Pharetra et ultrices neque ornare aenean euismod. Pretium viverra suspendisse potenti nullam ac tortor vitae. Morbi quis commodo odio aenean sed. At consectetur lorem donec massa sapien faucibus et. Nisi quis eleifend quam adipiscing vitae proin sagittis nisl rhoncus. Duis at tellus at urna condimentum mattis pellentesque. Vivamus at augue eget arcu dictum varius duis at. Justo donec enim diam vulputate ut. Blandit libero volutpat sed cras ornare arcu. Ac felis donec et odio pellentesque diam volutpat commodo. Convallis a cras semper auctor neque. Tempus iaculis urna id volutpat lacus. Tortor consequat id porta nibh.

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Hac habitasse platea dictumst vestibulum rhoncus est pellentesque. Amet dictum sit amet justo donec enim diam vulputate ut. Neque convallis a cras semper auctor neque vitae. Elit at imperdiet dui accumsan. Nisl condimentum id venenatis a condimentum vitae sapien pellentesque. Imperdiet massa tincidunt nunc pulvinar sapien et ligula. Malesuada fames ac turpis egestas maecenas pharetra convallis posuere. Et ultrices neque ornare aenean euismod. Suscipit tellus mauris a diam maecenas sed enim. Potenti nullam ac tortor vitae purus faucibus ornare. Morbi tristique senectus et netus et malesuada. Morbi tristique senectus et netus et malesuada. Tellus pellentesque eu tincidunt tortor aliquam. Sit amet purus gravida quis blandit. Nec feugiat in fermentum posuere urna. Vel orci porta non pulvinar neque laoreet suspendisse interdum. Ultricies tristique nulla aliquet enim tortor at auctor urna. Orci sagittis eu volutpat odio facilisis mauris sit amet.

Tellus molestie nunc non blandit massa enim nec dui. Tellus molestie nunc non blandit massa enim nec dui. Ac tortor vitae purus faucibus ornare suspendisse sed nisi. Pharetra et ultrices neque ornare aenean euismod. Pretium viverra suspendisse potenti nullam ac tortor vitae. Morbi quis commodo odio aenean sed. At consectetur lorem donec massa sapien faucibus et. Nisi quis eleifend quam adipiscing vitae proin sagittis nisl rhoncus. Duis at tellus at urna condimentum mattis pellentesque. Vivamus at augue eget arcu dictum varius duis at. Justo donec enim diam vulputate ut. Blandit libero volutpat sed cras ornare arcu. Ac felis donec et odio pellentesque diam volutpat commodo. Convallis a cras semper auctor neque. Tempus iaculis urna id volutpat lacus. Tortor consequat id porta nibh.

29 More Pages to Come in This Document. Get access to the complete answer.

More bchm482 BCHM 482 Proteomics and Metabolomics : Questions & Answers

Management

What are some recently-developed or potential future treatments for T1D? Choose one approach that you think might succeed; describe current progress; discuss potential advantages and obstacles? We have uploaded two files that you can use to ask this question. Do not give a lengthy introduction on T1 …

View Answer

BIO 102 Principles of Biology


Objective: To examine the effects of mutagenic agents on growth development of organism and to composed and argue a perspective on the topic of effect of mutagens on organism in a formal written paper
Overview
Ever since the discover of natural radiation by Becquerel in 1896, humans have a …

View Answer

SPOR4011 Introduction to Biomechanics

Task:
For this assignment you must answer 3 questions, each question has a maximum word limit of 500 words, any words over the 500 word limit for each question will not be answered and unused words from one question cannot be applied to another question. You must therefore write concisely and acc …

View Answer

Management

Title: Ecological study on the impacts of functional traits in seedlings of Eastern Australian tree/shrub species …

View Answer

Content Removal Request

If you are the original writer of this content and no longer wish to have your work published on Myassignmenthelp.com then please raise the
content removal request.

Choose Our Best Expert to Help You

Still in Two Minds? The Proof is in Numbers!

38983 Genuine Reviews With a Rating of 4.9/5.

Healthcare

Assignment: 4 Pages, Deadline:
11 days

The writing is really good and went beyond my expectation. All the requirements are met and the sources were used in the essay are a reliable and good …

User ID: 5***30 Canada

Biology

Home Work: 1 Page, Deadline:
21 days

great staff, nice people they submitted my work onetime and I had passed my unit

User ID: 4***37 Sydney, Australia

Electronics

Programming: 0 Pages, Deadline:
2 days

excellent! very fast speed and good work. i appreciate the hard work from your team. will use again

User ID: 7***68 Sydney, Canada

Accounting

Course Work: 3 Pages, Deadline:
12 days

Hi! Thanks, again for your help! Very professional writer and finished on time! Quality is the best

User ID: 3***99 Ottawa, Canada

Finance

Programming: 2 Pages, Deadline:
4 days

The best work! Thanks, again for your help! I got the grade that I wanted! Very professional and well-organized

User ID: 3***99 Ottawa, Canada

Accounting

Course Work: 2 Pages, Deadline:
5 days

I got the mark that I wanted. Thank you, again for your help. Well-developed and organized.

User ID: 3***99 Ottawa, Canada

Psychology

Assignment: 6 Pages, Deadline:
13 days

cant complain happy by the look of the assignment! I will give more feedback once grade received

User ID: 8***95 Ottawa, Ireland

Management

Essay: 3 Pages, Deadline:
2 days

Wonderful original work. I was so impressed and thankful for all of the help. This made my assignment so easy to submit.

User ID: 8***72 Washington Court House, United States

It Write Up

Assignment: 2 Pages, Deadline:
17 hours

I need an clear an rich picture with details and the event table with type external,temporal,state in events type and trigger,action,condition respone …

User ID: 7***16 Washington Court House, Australia

HRM

Home Work: 1.5 Pages, Deadline:
5 days

great score on this assignment, feedback was on time. the assignment was submitted within the stipulated time frame.

User ID: 6***66 Washington Court House, Saint LUCIA

Management

Home Work: 18 Pages, Deadline:
9 days

Excellent but a lot of Citation was needed. got passing marks but it was critically analyzed.

User ID: 8***44 United Kingdom, Great Britain

Finance

Course Work: 8 Pages, Deadline:
20 hours

The writer did an excellent job writing the APA research paper and this individual met my requirements. Thank you for doing extraordinary work!

User ID: 8***35 Saint Louis, United States

Management

Home Work: 1 Page, Deadline:
14 hours

great work but now i did not get marks from professor. I like your work.Great great great great great…….

User ID: 8***10 Surrey, Canada

Project Management

Thesis: 20 Pages, Deadline:
7 days

It was a pretty good assignment I hope that and the others will be like this one

User ID: 6***39 Surrey, Greece

Management

Assignment: 10 Pages, Deadline:
5 days

i always go back for the assignment !! they are the best amazing work so fast and on time !!

User ID: 7***36 Riyadh, Saudi Arabia

HRM

Essay: 1 Page, Deadline:
2 days

well-written discussion and completed before deadline. Excellent service by customer care team.

User ID: 7***17 Riyadh, Australia

Marketing

Assignment: 10 Pages, Deadline:
4 days

Thank you for this excellent and valuable fileI thank the expert for his professional efforts in preparing the fileI would also like to thank the team …

User ID: 9***3 Riyadh, Kuwait

Management

Programming: 8 Pages, Deadline:
16 days

Hi thank you once again excellent service and timely, thank you for the hard work please thank the writer much appreate the effort involved

User ID: 7***88 Melbourne, Australia

It Write Up

Assignment: 5 Pages, Deadline:
5 days

The assignment was done on time and received good feedback from the teacher. Thank you team for helping out

User ID: 3***28 Melbourne, United States

Programing

Programming: 3 Pages, Deadline:
3 days

Excellent work for this particular assignment, and it is extremely helpful thanks!

User ID: 8***44 Melbourne, United States

Healthcare

Assignment: 4 Pages, Deadline:
11 days

The writing is really good and went beyond my expectation. All the requirements are met and the sources were used in the essay are a reliable and good …

User ID: 5***30 Canada

Biology

Home Work: 1 Page, Deadline:
21 days

great staff, nice people they submitted my work onetime and I had passed my unit

User ID: 4***37 Sydney, Australia

Electronics

Programming: 0 Pages, Deadline:
2 days

excellent! very fast speed and good work. i appreciate the hard work from your team. will use again

User ID: 7***68 Sydney, Canada

Accounting

Course Work: 3 Pages, Deadline:
12 days

Hi! Thanks, again for your help! Very professional writer and finished on time! Quality is the best

User ID: 3***99 Ottawa, Canada

Finance

Programming: 2 Pages, Deadline:
4 days

The best work! Thanks, again for your help! I got the grade that I wanted! Very professional and well-organized

User ID: 3***99 Ottawa, Canada

Accounting

Course Work: 2 Pages, Deadline:
5 days

I got the mark that I wanted. Thank you, again for your help. Well-developed and organized.

User ID: 3***99 Ottawa, Canada

Psychology

Assignment: 6 Pages, Deadline:
13 days

cant complain happy by the look of the assignment! I will give more feedback once grade received

User ID: 8***95 Ottawa, Ireland

Management

Essay: 3 Pages, Deadline:
2 days

Wonderful original work. I was so impressed and thankful for all of the help. This made my assignment so easy to submit.

User ID: 8***72 Washington Court House, United States

It Write Up

Assignment: 2 Pages, Deadline:
17 hours

I need an clear an rich picture with details and the event table with type external,temporal,state in events type and trigger,action,condition respone …

User ID: 7***16 Washington Court House, Australia

HRM

Home Work: 1.5 Pages, Deadline:
5 days

great score on this assignment, feedback was on time. the assignment was submitted within the stipulated time frame.

User ID: 6***66 Washington Court House, Saint LUCIA

Management

Home Work: 18 Pages, Deadline:
9 days

Excellent but a lot of Citation was needed. got passing marks but it was critically analyzed.

User ID: 8***44 United Kingdom, Great Britain

Finance

Course Work: 8 Pages, Deadline:
20 hours

The writer did an excellent job writing the APA research paper and this individual met my requirements. Thank you for doing extraordinary work!

User ID: 8***35 Saint Louis, United States

Management

Home Work: 1 Page, Deadline:
14 hours

great work but now i did not get marks from professor. I like your work.Great great great great great…….

User ID: 8***10 Surrey, Canada

Project Management

Thesis: 20 Pages, Deadline:
7 days

It was a pretty good assignment I hope that and the others will be like this one

User ID: 6***39 Surrey, Greece

Management

Assignment: 10 Pages, Deadline:
5 days

i always go back for the assignment !! they are the best amazing work so fast and on time !!

User ID: 7***36 Riyadh, Saudi Arabia

HRM

Essay: 1 Page, Deadline:
2 days

well-written discussion and completed before deadline. Excellent service by customer care team.

User ID: 7***17 Riyadh, Australia

Marketing

Assignment: 10 Pages, Deadline:
4 days

Thank you for this excellent and valuable fileI thank the expert for his professional efforts in preparing the fileI would also like to thank the team …

User ID: 9***3 Riyadh, Kuwait

Management

Programming: 8 Pages, Deadline:
16 days

Hi thank you once again excellent service and timely, thank you for the hard work please thank the writer much appreate the effort involved

User ID: 7***88 Melbourne, Australia

It Write Up

Assignment: 5 Pages, Deadline:
5 days

The assignment was done on time and received good feedback from the teacher. Thank you team for helping out

User ID: 3***28 Melbourne, United States

Programing

Programming: 3 Pages, Deadline:
3 days

Excellent work for this particular assignment, and it is extremely helpful thanks!

User ID: 8***44 Melbourne, United States

Have any Query?
BCHM 482 Proteomics and Metabolomics : Integrative Analysis
Scroll to top

GET THIS ANSWER FROM EXPERTS NOW

WhatsApp
Hello! Need help with your assignments? We are here